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The attenuation of diabetes-induced osteoporosis through ANGPTL8 knockout. ( A ) Schematic representation of the diabetic osteoporosis model construction. ( B ) FBG levels in mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( C ) Body weight measurements of mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( D ) ELISA analysis for quantifying ANGPTL8 levels in mouse plasma. ( E <t>)</t> <t>Micro-CT</t> imaging of mouse femurs. ( F - J ) Analysis of Micro-CT data for bone mineral density (BMD), bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) in mouse femurs. Data are presented as mean ± SD; ( N = 5). ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001
Micro Computed Tomography Micro Ct Scanning, supplied by Revvity, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer controlled context conditioning system  (Med Associates Inc)
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The attenuation of diabetes-induced osteoporosis through ANGPTL8 knockout. ( A ) Schematic representation of the diabetic osteoporosis model construction. ( B ) FBG levels in mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( C ) Body weight measurements of mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( D ) ELISA analysis for quantifying ANGPTL8 levels in mouse plasma. ( E <t>)</t> <t>Micro-CT</t> imaging of mouse femurs. ( F - J ) Analysis of Micro-CT data for bone mineral density (BMD), bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) in mouse femurs. Data are presented as mean ± SD; ( N = 5). ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001
Computer Controlled Context Conditioning System, supplied by Med Associates Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The attenuation of diabetes-induced osteoporosis through ANGPTL8 knockout. ( A ) Schematic representation of the diabetic osteoporosis model construction. ( B ) FBG levels in mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( C ) Body weight measurements of mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( D ) ELISA analysis for quantifying ANGPTL8 levels in mouse plasma. ( E <t>)</t> <t>Micro-CT</t> imaging of mouse femurs. ( F - J ) Analysis of Micro-CT data for bone mineral density (BMD), bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) in mouse femurs. Data are presented as mean ± SD; ( N = 5). ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001
Computer Monitor Display, supplied by Cambridge Research Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The attenuation of diabetes-induced osteoporosis through ANGPTL8 knockout. ( A ) Schematic representation of the diabetic osteoporosis model construction. ( B ) FBG levels in mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( C ) Body weight measurements of mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( D ) ELISA analysis for quantifying ANGPTL8 levels in mouse plasma. ( E <t>)</t> <t>Micro-CT</t> imaging of mouse femurs. ( F - J ) Analysis of Micro-CT data for bone mineral density (BMD), bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) in mouse femurs. Data are presented as mean ± SD; ( N = 5). ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001
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computed tomography myocardial perfusion imaging  (Canon inc)
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The attenuation of diabetes-induced osteoporosis through ANGPTL8 knockout. ( A ) Schematic representation of the diabetic osteoporosis model construction. ( B ) FBG levels in mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( C ) Body weight measurements of mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( D ) ELISA analysis for quantifying ANGPTL8 levels in mouse plasma. ( E <t>)</t> <t>Micro-CT</t> imaging of mouse femurs. ( F - J ) Analysis of Micro-CT data for bone mineral density (BMD), bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) in mouse femurs. Data are presented as mean ± SD; ( N = 5). ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001
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statistical software computer program version 20  (SPSS Inc)
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The attenuation of diabetes-induced osteoporosis through ANGPTL8 knockout. ( A ) Schematic representation of the diabetic osteoporosis model construction. ( B ) FBG levels in mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( C ) Body weight measurements of mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( D ) ELISA analysis for quantifying ANGPTL8 levels in mouse plasma. ( E <t>)</t> <t>Micro-CT</t> imaging of mouse femurs. ( F - J ) Analysis of Micro-CT data for bone mineral density (BMD), bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) in mouse femurs. Data are presented as mean ± SD; ( N = 5). ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001
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micro computed tomography scanning  (Revvity)
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Experimental phantom composition. (a) Phantoms’ background absorption and reduced scattering coefficients. (b) Set of phantom molds with capillary tubes at different depths. (c) Capillary tube depth confirmation <t>using</t> <t>micro-computed</t> tomography.
Micro Computed Tomography Scanning, supplied by Revvity, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Experimental phantom composition. (a) Phantoms’ background absorption and reduced scattering coefficients. (b) Set of phantom molds with capillary tubes at different depths. (c) Capillary tube depth confirmation <t>using</t> <t>micro-computed</t> tomography.
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The attenuation of diabetes-induced osteoporosis through ANGPTL8 knockout. ( A ) Schematic representation of the diabetic osteoporosis model construction. ( B ) FBG levels in mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( C ) Body weight measurements of mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( D ) ELISA analysis for quantifying ANGPTL8 levels in mouse plasma. ( E ) Micro-CT imaging of mouse femurs. ( F - J ) Analysis of Micro-CT data for bone mineral density (BMD), bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) in mouse femurs. Data are presented as mean ± SD; ( N = 5). ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: ANGPTL8 accelerates bone loss in diabetic mice by promoting osteoclastic differentiation and inhibiting osteoblastic differentiation through AMPK pathway-mediated metabolic reprogramming

doi: 10.1007/s00018-025-06077-x

Figure Lengend Snippet: The attenuation of diabetes-induced osteoporosis through ANGPTL8 knockout. ( A ) Schematic representation of the diabetic osteoporosis model construction. ( B ) FBG levels in mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( C ) Body weight measurements of mice at the onset (0 weeks) and conclusion (20 weeks) of the experiment. ( D ) ELISA analysis for quantifying ANGPTL8 levels in mouse plasma. ( E ) Micro-CT imaging of mouse femurs. ( F - J ) Analysis of Micro-CT data for bone mineral density (BMD), bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) in mouse femurs. Data are presented as mean ± SD; ( N = 5). ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The trabecular bone microarchitecture in the right femur of mice was assessed using Micro-computed tomography (Micro-CT) scanning (Quantum GX2; Perkin Elmer).

Techniques: Knock-Out, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Micro-CT, Imaging

Experimental phantom composition. (a) Phantoms’ background absorption and reduced scattering coefficients. (b) Set of phantom molds with capillary tubes at different depths. (c) Capillary tube depth confirmation using micro-computed tomography.

Journal: Journal of Biomedical Optics

Article Title: Evaluation of analytical models to estimate depth of fluorescence objects in biological media

doi: 10.1117/1.JBO.31.2.026003

Figure Lengend Snippet: Experimental phantom composition. (a) Phantoms’ background absorption and reduced scattering coefficients. (b) Set of phantom molds with capillary tubes at different depths. (c) Capillary tube depth confirmation using micro-computed tomography.

Article Snippet: Once solidified, the phantoms immediately underwent fluorescence imaging followed by confirmation of fluorescent inclusion (i.e., capillary tube) depth by micro-computed tomography scanning ( 80 μ m resolution, Quantum GX3, Revvity, Waltham, Massachusetts, United States); shows an example of depth confirmation for a capillary tube of 7 mm nominal depth.

Techniques: Micro-CT